Electrophoretic studies of sonic extracts of Proteus vulgaris. I. Effect of growth environment on electrophoretic patterns.
نویسنده
چکیده
The analytical electrophoretic pattern of the buffer-soluble proteins extracted from bacterial cells is both characteristic and descriptive of the organism (Hess et al., 1957; Wagman et al., 1958; Rodenberg, 1958). Further, a relatively large fraction of the material of the cell can be studied by the electrophoretic method. Hess et al. (1957) using successive extractive procedures on three strains of Streptococcus pyogenes obtained electrophoretic data on as much as 84 per cent of the dry weight of the cell and Rodenberg (1958) reported electrophoretic data on Proteus vulgaris strain OX-19 which were descriptive of 70 per cent of the total cellular nitrogen. It was the object of this study to determine whether altered environmental situations would produce sufficiently large qualitative or quantitative differences in the soluble proteins of the cell so that they might be detected electrophoretically. In part, some of these studies were reported previously (Rodenberg, 1957) and Wagman et al. (1958) found some corroborative results.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 77 5 شماره
صفحات -
تاریخ انتشار 1959